Construction of artificial hydantoinase by molecular imprinting technique

摘要

Molecular imprinting technology has developed into a viable approach for mimicking natural recognition entities, such as antibodies and biological receptors during the past three decades. Now, one of the most intriguing challenges for the use of molecularly imprinted materials is their application as enzyme mimics in synthesis and catalysis. Given the combination of high selectivity and robustness that can be accomplished with the imprinted materials, their use in demanding process may find many niches, such as operation at elevated temperature and pressure, reaction under acidic or basic conditions, and use in organic solvent. As a valuable enzyme for the production of optically pure D-and L-arnino acids, hydantoinases catalyses the reversible hydrolytic ring cleavage of hydantoin or 5'-substituted hydantoins. but the high cost and poor performance in organic solvent limit the large-scale application of hydantoinases in industry. In this research we constructed an artificial hydantoinase by molecular imprinted technology, vinylimidazole-divalent transition metal ion-MAA complex was used to mimic the active center of hydantoinases. The polymer catalyst obtained show significantly catalytic activity to the hydrolysis of hydantoin, and the catalytic activity of the polymer catalyst was improved significantly by molecular imprinting procedure. The molecular imprinted catalyst (MIC) also shows significant substrate selectivity.