Generation of a novel selenium-containing scFv abzyme with antioxidant effect

摘要

Glutathione peroxidase (EC 1.11.1.9, GPX) is one of the most important antioxidant enzymes in organism, which distributes extensively in cell, blood and tissue. GPX prevents the organism from the damage of free radicals by scavenging hydroperoxide and lipid hydroperoxide, and maintains the normal physiological functions of the cell and tissues. It is in close relationship with many diseases, but for the disadvantages of native GPX, e.g. limited availability, it is hard to be. used extensively. So many artificial mimics of GPX have been made. A novel Se-ScFv abzyme was generated and its antioxidant effect was studied in this paper. UV radiation of sunlight has been associated with an increased incidence of skin cancer. UVB can induce cellular injury such as DNA damage, lipid peroxidation and apoptosis. So in the present study UVB was used to generate lipid peroxidation. and the MDA content, LDH content in culture medium, MTT reducation content and DNA fragmentation were used to observe the damage from UVB and protection of Se-2F3-ScFv to epidermal cells against UVB.