Total internal reflection imaging ellipsometry (TIRIE) biosensor sensitivity improvement with low noise imaging device

摘要

The biosensors with properties of real-time, high throughput and label-free are more and more popular recently, in which the biosensor based on the total internal reflection imaging ellipsometry (TIRIE) is a novel imaging detector for protein interaction processes. In previous work, three techniques are introduced to improve the performance of the biosensor including polarization setting optimization, spectroscopic light source application and low noise CCD detector adoption. In this paper, the effect of the low noise CCD detector technology on the sensitivity and detection limit improvement is analyzed. An obvious improvement of 10 time increase in the sensitivity and SNR, and 50 times lower concentration in the detection limit is obtained by optimization.