Smart pH Cuvette for optical monitoring of pH of biological samples

摘要

A Smart pH Cuvette is developed by coating the inner surface with pH sensitive thin film. The coating is a hydroscopic sol-gel material doped with colorimetric pH indicator dye sensitive to the pH of analyte solutions in biological range. Ocean optics miniaturized spectrometers are used for signal detection and analysis, along with multimode optical fibers. This new pH sensing arrangement yields an inexpensive solution for monitoring the pH of samples for biological applications. The Smart pH Cuvettes provide a resolution of 0.01 pH units, an accuracy of 1% of the reading, and 90% response in less than 10 seconds.