PRELIMINARY GENOTYPING FOR COX-2-765G>C BASED ON SNP DETECTION WITH MAGNETIC NANOPARTICLES

摘要

Single nucleotide polymorphisms (SNPs) are the most frequent forms of sequence variations in the human genome. They have many advantages such as large quantity, wide distribution, fast detection, easy for genotyping and so oa Magnetic nanoparticles (MNPs) have been widely applied nowadays. We introduced a high-throughput SNP genotyping method based on MNPs and dual-color fluorescence hybridization. Amplified PCR products were captured on MPs and interrogated by hybridization with a pair of dual-color probes to determine SNP, and then the genotype of each sample can be simultaneously identified by scanning the microarray printed with the denatured fluorescent probes. Without any purification and concentration of PCR products, the approach reported here should be a simple, sensitive, high-throughput and high accurate genotyping method. The cyclooxygenase-2-765G>C polymorphisms of seventy different samples were detected and the genotypes for the locus were discriminated easily. The genotyping results were additionally validated by sequencing.