High-resolution three-dimensional structure determination of large macromolecular assemblies by electron cryomicroscopy --- cytoplamics polyhedrosis virus reconstruction at 8 A

摘要

The explosion of protein sequence data resulting from large-scale genome sequencing has created unprecedented challenges and opportunities for electron microscopists and computational scientists. Traditional structural approaches, such as X-ray crystallography and NMR, which are the primary tools for determining atomic structures of macromolecules, can no longer keep pace with this rapid development, thus posing a need for novel approaches to high-resolution structural determination, particular for supramolecular assemblies that are difficult to study by traditional means.