Simple Immobilization Method of Protein and Adhesive Cell on Functionalized Surface

摘要

This study presented simple surface modification for the immobilization of biomolecules such as proteins and cells in order to integrated with microfluidic system. Firstly, the fabrication of thin film composed of glycidyl methacrylate (GMA) that amine- attractive epoxide monomer was fabricated with photopolymerization. The orthogonal surface using simple micromolding in capillaries (MIMIC) was obtained. One region included polyethylene glycol (PEG) microstructures that prevent nonspecific binding of proteins and cells. The other region was the epoxide surface that promoted protein and cell immobilization. The orthogonal difference in surface functionality between the epoxide region and background PEG show strong possibility of simple patterning of biomolecules. In addition, the size, shape, height of pattern could be easily controlled by PDMS mold and spinning condition. It will be useful platform technology for the constrcution of organic material patterning, protein array, and cell.