Allelopathy of Rehmannia Glutinosa on sesame

摘要

Rehmannia glutinosa Libosch, a well known herb for Chinese traditional medicine and Sesame are two main crops in northwestern Henan, china. It has been known that however a rotation of these two crops, eg. cultivation of seaseme proceeded with Rehmannia glutinosa Libosch leads to decreased yield and poor quality of sesame In order to identify the compound or allelochemicals which inhibit the growth of sesame, the effect of the crude, tissue extractions of Rehmannia glutinosa on the cultivation of sesame was analysized. We found most allelochemicais exist in the Ethyl acetate extraction of the leaves. Futher fractionation of the leave extraction on silica gel column chromatography followed by GCMS (gas chromatography mass spectrometry), we for the first time identified lauric acid and 2,6-Ditertbutyl phenol as two main alllelochemicals, which inhibit the seed germination of sesame. The identification of the allelochemicals in this study pave the way for further characterization of the molecular interaction of allelochemicals from the proceeding crop Rehmannia glutinosa Libosch, the soil and the succeeding crop sesame, which could be beneficial for improvement of the crop rotation between rehmannia glutinosa and sesame.