TISSUE EXPRESSION AND CONSTRUCTION OF PROKARYOTIC EXPRESSION SYSTEM OF GALLINACIN-2

摘要

IntroductionThe alarming increase of drug-resistant bacteria and banning of antimicrobial growth promoters world wide make the search for novel means of fighting bacterial infections and promoting animal growth imperative (Van den Bogaard and Stobberingh, 2000). The beta-defensin may be the ideal substitutes for bactericidal agents and antimicrobial growth promoters. Gallinacins (Gal) in poultry are functional equivalents of mammalian beta-defensins, which constitute an integral component of the innate immune system. The reported biological activities of beta-defensin included anti-microbial, antiviral, anti-tumor, immunomodulation effects, and growth promotation (Ganz, 2003; Peng et al., 2007). GaUinacin-2 (Gal-2), expressed in the respiratory tract and bone marrow of chicken, is abundant in cells that are involved in the innate immune response against microbial infections (Sugiarto and Yu, 2004). To our knowledge, it has been mentioned rarely that the tissue expression of Gal-2 of single comb white leghorn (SCWL) and the prokaryotic expression of Gallinacin-2, especially in pMAL-c2X system. The objective of this study was research the tissue expression of Gal-2 of SCWL and construction of prokaryotic expression system of Gallinacin-2 using pMAL-c2X.