Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola

摘要

The methods of degenerate PCR and DNA walking were used to clone a fragment of 4 274bp nucleotides from A. salicacla strain YAASM0711. With sequence alignment and prediction, one mating gene encoding pheromone receptor was found to be 1194bp nucleotides long and included four introns and five extrons with the lengths of 217bp, 113bp, 67bp, 138bp and 449bp respectively. The spliced open reading frame (ORF) contains 984bp nucleotides encoding 327 amino acid residues, and includes seven transmembrane domains, similar to those from Coprinus cinerea and Laccaria bicolor pheromone receptors. The special primers designed flanking the ORF can separate B1 from B2 mating type strains, indicating that they can be used to distinguish mating types among strains.