Quantified characterization of dermal collagen structure in human cutaneous scars from second harmonic generation imaging

摘要

In this paper, high resolution images of collagens based on second harmonic generation (SHG) were obtained in the dermis of different scar tissues and surrounding considered uninjured skins, demonstrating differences in their microstructures. In order to quantitatively analyze their structural features, Fourier analysis was applied for the SHG images to compare the alteration in collagen orientation of different scars. Moreover, depth dependant decay of SHG signals was also analyzed to provide more credible information. Our results show that the different types of scar tissues can be successfully distinguished with the two combined methods, which will help to determine the most appropriate clinical treatment strategy.