Effect of a thin blood layer on fluorescence spectroscopy

摘要

When performing intravascular spectroscopy there is always the possibility that there will be a thin layer of blood between the probe and the vessel wall. This will affect measurements espescially below 600 nrn where blood absorption is strong (important for fluorescence and Raman measurements) and above 1400 nm where the water absorption is strong. Between these two regions (the theraputic window) the absorption is fairly low and can be neglected. This article explores the possibility of performing measurements outside the optical window by minimizing blood layer and increasing the excitation signal.