Hydrolysis of Paper Sludge Using Mixed Cellulase System: Enzymtic Hydrolysis of Paper Sludge

摘要

The effects of water soluble materials in paper sludge (sludge for short) on cellulase and β-glucosidase activities and the effects of pre-drying of sludge and initial glucose concentrations on the enzymatic sludge hydrolysis were studied. The optimi/ation of enzyme system for hydrolysis of the sludge for production of glucose was also made. Water soluble materials in the sludge showed stimulatory effect on carboxymethyl cellulose (CMC) activity, inhibitory effect on filter paper (FP) activity and no effect on avicelase and P-glucosidase activities. Re-wetted sludge after drying showed 64% decrease in glucose production than that for the non-dried sludge in hydrolysis. The initial glucose concentrations of 5, 10 and 20 g/l resulted in 9.5%, 20.4% and 39.2% decreases in glucose production, respectively, after 16 h of hydrolysis. CMC and β-glucosidase activities at 5 and 10, 10 and 10, 10 and 20 U/ml were optimal for hydrolysis of 5, 10 and 20% of sludge, respectively.