Expression of a Proapoptotic Bcl-xl by Low-energy Laser Irradiation in the Amyloid Beta 25-35 Induced Apoptosis of PC12 Cells

摘要

Among the many physiological effects of low-energy laser irradiation, anti-apoptosis functions have been reported. In our experiments A-beta was used to induce cells apoptosis and then the cells were dealt with low-energy laser irradiation (LELI), and finally the LELI effects to apoptosis were detected. The morphology of apoptotic cells was evaluated by fluorescence microscope. The molecular level of apoptosis was detected by quantifying the Bcl-xl mRNA expression using a new highly sensitive and quantitative polymerase chain reaction (QT-PCR) technique. The results show that the Bcl-xl mRNA expression went variety with different treatments to the cells. That indicates that the Bcl-xl is involved in the LELI effect of the A β -induced apoptosis.