A non-destructive diffuse reflectance calibration-free method to determine optical parameters of biological tissues

摘要

A method for non-destructive determination of absorption and transport (reduced) scattering coefficients of turbid media (biological tissue, first of all) is presented. It refers to the spatially resolved diffuse reflectance techniques with optical fiber probe. The method is based on a more accurate (in comparison with diffusion) two parameters kinetic light propagation model and a special two step non-analog Monte Carlo technique, it involves no additional parameters and uses no assumptions about spectral dependencies of the coefficients, allows application of monochrome sources and probes with minimally possible number of reading fibers (only 2), goes without calibration phantoms and measurements. Numerical and experimental testing have showed the measured coefficients provide a good prediction of both light reflection and penetration fields in semi-infinite homogeneous media with low-mid absorption.