Methods for measuring changes in optical properties of wound tissue and correlating near infrared absorption (fNIR) and diffuse reflectance spectroscopy scattering (DRS) with tissue neovascularization and collagen concentration to determine whether wound is healing

摘要

Optical changes of tissue during wound healing measured by Near Infrared and Diffuse Reflectance Spectroscopy are shown to correlate with histologic changes. Near Infrared absorption coefficient correlated with blood vessel in-growth over time, while Diffuse Reflectance Spectroscopy (DRS) data correlated with collagen concentration. Changes of optical properties of wound tissue at greater depths are also quantified by Diffuse Photon Density Wave (DPDW) methodology at near infrared wavelengths. The diffusion equation for semi-infinite media is used to calculate the absorption and scattering coefficients based on measurements of phase and amplitude with a frequency domain or time domain device. An increase in the absorption and scattering coefficients and a decrease in blood saturation of the wounds compared to the non wounded sites was observed. The changes correlated with the healing stage of the wound. The methodologies used to collect information regarding the healing state of a wound may be used to clinically assess the efficacy of wound healing agents in a patient (e.g., a diabetic) and as a non-invasive method to detect the progress of wound healing, particularly chronic wounds due to diabetes. The methodology applies to ischemic environments, impaired healing states, and emerging subsurface tissue deterioration, such as in pressure ulcers, venous ulcers, and ubiquitous ulcers.