METHODS FOR MEASURING CHANGES IN OPTICAL PROPERTIES OF WOUND TISSUE AND CORRELATING NEAR INFRARED ABSORPTION (FNIR) AND DIFFUSE REFLECTANCE SPECTROSCOPY SCATTERING (DRS) WITH TISSUE NEOVASCULARIZATION AND COLLAGEN CONCENTRATION TO DETERMINE WHETHER WOUND IS HEALING

摘要

Optical changes of tissue during wound healing measured by Near Infrared andDiffuse Reflectance Spectroscopy are shown to correlate with histologicchanges. Thediffusion equation for semi-infinite media is used herein to calculate theabsorption andscattering coefficients of wounds based on measurements of phase and amplitudewith afrequency domain or time domain device. An increase in the absorption andscatteringcoefficients and a decrease in blood saturation of the wounds compared to thenon woundedsites was observed. The changes correlated with the healing stage of thewound. Themethodologies used to collect information regarding the healing state of awound may be usedto clinically assess the efficacy of wound healing agents in a patient (e.g.,a diabetic) and as anon-invasive method to detect the progress of wound healing.