Evaluation of the cell depleted layer of blood flow in vitro using confocal microscopic PIV method

摘要

In the present study, we employed the laser scanning confocal microscope to image entire blood flow with accurate red blood cell imaging of 0.001 mm spatial resolution. In vitro blood flow of rat with different hematocrit ratios was simulated inside a 100and 300-micron opaque tube. The scanning rate of confocal microscope was 30 fps with 500 x 500 pixels of image. As a result, we can obtain clear images of RBCs to which is enough to be used as tracer particle directly to get the velocity vector field of blood flow by performing particle image velocimetry (PIV) technique non-invasively. Based on the present novel optical application, we can easily indicate the presence of cell depleted layer of blood flow in vitro and its boundaries.