Comparison of the Efficiency of Different Methods for the Lysis of Cells in Lab-on-Chip Systems

摘要

Quantitative or qualitative examination of DNA has enormous impact on medical, forensic, or genealogical, analysis. The macro as well as micro world knows a panel of different methods for cell lysis that has to happen prior to the purification of the DNA. To our knowledge the efficiency of those protocols as well as the purity/amplifiability of extracted DNA has not been compared for a Lab-on-a-Chip application so far. To address this issue, human HeLa cells were lysed on-chip by chemical, enzymatic, thermal or mechanical treatment, or by application of strong pH gradients. These tests are currently complemented by approaches using exposure to strong electrical fields, ultrasonic treatment or radio frequencies. In the first instance quantity and purity of DNA was inspected by agarose gel electrophore-sis. Up to now, the chemical lysis by applying Guanidinium SCN and the electrochemical lysis represent to be the best DNA isolation protocols in our chip-based test system.