Formulation Optimization for the Nanoparticles-in-Microsphere Hybrid Oral Delivery Systems Using Factorial Design

摘要

The objective of the present study was to optimize the processing parameters for nanoparticles-in-microsphere oral delivery system (NiMOS) and to mathematically relate the process parameters with the selected attribute (particle size) of the hybrid system. Fluorescein Isothiocyanate (FITC) labeled gelatin nanoparticles were encapsulated in poly(epsilon caprolactone) (PCL) micropshere and this system was optimized for particle size using a full 33 factorial design. Particles with size less than 10 micrometer were processed based on the results obtained from factorial design. These particles can be used for the oral delivery of macromolecules such as peptide, proteins, and other therapeutics.