Enhancing stimulated emission-based fluorescence detection with interferometric setup

摘要

Fluorescence lifetime imaging microscopy (FLIM) can reveal important biological information and recently stimulated emission (SE) has been applied in FLIM to improve the spatial resolution of micrographs and detect fluorophore over a long working distance. An issue with SE is that the SE signal is much weaker than the probe laser beam that is used to generate the SE, therefore the signal to background ratio is low. Here we demonstrate using interferometric setup to decrease this background laser intensity, thus achieving higher S/N ratio and dye concentration detection sensitivity in SE microscopy.