PharmaSat: Drug dose response in microgravity from a free-flying integrated biofluidic/optical culture-and-analysis satellite

摘要

We designed, built, tested, space-qualified, launched, and collected telemetered data from low Earth orbit from PharmaSat, a 5.1-kg free flying "nanosatellite" that supported microbial growth in 48 microfluidic wells, dosed microbes with multiple concentrations of a pharmaceutical agent, and monitored microbial growth and metabolic activity using a dedicated 3-color optical absorbance system at each microwell. The PharmaSat nanosatellite comprised a structure approximately 10×10×35 cm, including triple-junction solar cells, bidirectional communications, power-generation and energy-storage system, and a sealed payload 1.2-L containment vessel that housed the biological organisms along with the fluidic, optical, thermal, sensor, and electronic subsystems. Growth curves for 5. cerevisiae (Brewer's yeast) were obtained for multiple concentrations of the antifungal drug voriconazole in the microgravity conditions of low Earth orbit. Corresponding terrestrial control experiments were conducted for comparison.