Probabilistic reachability analysis of the tap withdrawal circuit in caenorhabditis elegans

摘要

We present a probabilistic reachability analysis of a (nonlinear ODE) model of a neural circuit in Caeorhabditis elegans (C. elegans), the common roundworm. In particular, we consider Tap Withdrawal (TW), a reflexive behavior exhibited by a C. elegans worm in response to vibrating the surface on which it is moving. The neural circuit underlying this response is the subject of this investigation. Specially, we perform bounded-time reachability analysis on the TW circuit model of Wicks et al. (1996) to estimate the probability of various TW responses. The Wicks et al. model has a number of parameters, and we demonstrate that the various TW responses and their probability of occurrence in a population of worms can be viewed as a problem of parameter uncertainty. Our approach to this problem rests on encoding each TW response as a hybrid automaton with parametric uncertainty. We then perform probabilistic reachability analysis on these automata using a technique that combines a δ-decision procedure with statistical tests. The results we obtain are a significant extension of those of Wicks et al. (1996), who equip their model with fixed parameter values that reproduce a single TW response. In contrast, our technique allow us to more thoroughly explore the models parameter space using statistical sampling theory, identifying in the process the distribution of TW responses. Wicks et al. conducted a number of ablation experiments on a population of worms in which one or more of the neurons in the TW circuit are surgically ablated (removed). We show that our technique can be used to correctly estimate TW response-probabilities for four of these ablation groups. We also use our technique to predict TW response behavior for two ablation groups not previously considered by Wicks et al.