Elucidating the role of integrin o5 in mediating the therapeutic potency of circulating angiogenic cells cultured on collagen matrix

摘要

The culture of PBMCs on a collagen matrix enhances their angiogenic potential, at least in part, through the regulation of integrin a5. While a5 is not a collagen-binding protein, it is still affected by collagen matrix, which was seen through mRNA and protein expression. Blocking the activity of a5 in cmCACs reversed the improvements, observed in vitro and in vivo, in their function and pro-angiogenic effects. This study also presents a possible new way to increase the therapeutic effect of cmCACs by use of angiopoietin-1. Overall, we provide insight into novel mechanisms for improving the function of therapeutic CACs.