Isolation of Secondary Metabolite Compounds and Antibacterial Activities Tests From Hexane Extract of Stem Bark Melochia umbellate (Houtt) Stapf var. degrabrata K

摘要

This research aims to determine the content of secondary metabolite compounds and antibacterial activity of stem bark extract Melochia umbellate (Houtt) Stapf var. degrabrata. Samples of M. umbellate stem bark were extracted by meseration using methanol solvent. Separation and purification is done by partitioning, fractionation with chromatography, and recrystallization. Antibacterial activity test of hexane extract and third isolate from bark of M. umbellate was done by agar diffusion method against bacterium Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Phytochemical test results showed that the hexane extract of bark M. umbellate compounds containing alkaloids and triterpenoids. Isolates of compound D is a triterpenoid group compound, while FKa and FKb compounds are steroid group compounds. The hexane extract had the highest antibacterial activity against B. subtilis bacteria with inhibitory zone diameter 12,0 mm. Isolates of compound D have a weak inhibitory effect on all test bacteria. FKa compound isolates had the highest inhibition against B. subtilis and S. aureus bacteria with inhibitory zone diameters of 18,0 mm and 13,0 mm respectively. Whereas FKb compound isolates have the highest inhibitory effect against B. subtilis bacteria with inhibitory zone diameter 12,0 mm. The results of the test show that FKa compound from bark of M. umbellate has the potential to be antibacterial because the compound is able to inhibit bacterial growth with > 14 mm obstacle zone, especially against B. subtilis bacteria.