Spectropolarimetry diagnostics of cervical cytological smears for availability of papillomavirus

摘要

The purpose of the work was to demonstrate the possibility of optical diagnosis of cytological smears of the cervix for the presence of HPV using spectral and polarization methods. Comparison of cervical specimens with and without coilocytosis, irradiated with a range-shifted polarized radiation, showed significant differences in the values of linear dichroism and its spectral dependences. The difference between the coilocyte response characteristic in the range 395-415 nm was calculated using computer programs. As a result, the use of spectropolarization studies and the fluorescence method will improve the accuracy of patient selection for a costly procedure - high-carcinogenic DNA diagnostics of HPV by the standard method of polymerase chain reaction.