Synthesis and characterization of the new copolymer FOL FOL-PEG PEG-g-PEI PEI-GAL as a novel carrier for tumor targeted gene delivery

摘要

The ideal gene carriers requires both safety and tranfection efficiency. Polyethyleneimine is a well known cationic polymer which has high positive surface charge for condensing plasmid DNA. However it is cytotoxic in many cell lines and non biodegradable. To overcome the disadvantages, we prepared the copolymer "FOL-PEG-g-PEI-GAL". This conjugated copolymer was characterized using H1-NMR and FTIR spectrometry . The molecular weight and the zeta potential of this copolymer was compared to PEI .The FOL-PEG-g-PEI-GAL has zeta potential of +17.83 my. And effectively condensed plasmid DNA into nanoparticles due to proper N/P ratio.