Formation of network activity modification toward in vitro spatial-temporal memory via hippocampus-cortex co-culture

摘要

In vitro cultures have long been used to investigate basic properties of neuronal networks. In this study, we ask the question ifneuronal networks without functional circuits are capable of long-term memories. To answer the question, a hippocampal-corticalco-culture model was used. Our co-culture model was developed by connecting dissociated hippocampal-cortical networksthrough micro-tunnel device. Memory was designed as spatial-temporal events as series of electrical stimuli given first in oneneuronal network, followed by series of stimuli in another network with a delay of time. Our results showed modification inevoked response by electrical stimulation, and lasted in trails after several minutes, suggesting possibility of spatial-temporalmemory ability in basic neuronal networks.