CXCR4 Proteoliposomes Cell Free Expression and SPRi Ligand Binding

摘要

SYNTHELIS has developed a Cell Free expression platform to express functional membrane proteins (GPCRs, Ion Channels, Transporters, Receptors...) directly embedded into customized liposomes to provide the adapted environment for the target. Here, CXCR4 chemokine receptor has been expressed in Synthelis cell free system as proteoliposomes and its binding properties were validated using Horiba Scientific SPRi platform. Three different ligands (a chemokine, a small peptide and a small molecule) binding three different sites on CXCR4 has been used. All of them shown specific and dose-dependent response signal.