Reactive Oxygen Species, Ascorbic Acid and Glutathione to Ozone Stress in Soybean (Glycine max) Leave

摘要

Open-top chambers were used to investigate the mechanism of antioxidants to eliminate reactive oxygen species and the impacts on growth and development of plants under troposphere O3 stress. The results indicated that, compared to control, the O3 concentration of O3-1 (80±10nL·L-1) and O3-2 (110±10nL·L-1) induced an increase on malondialdehyde content, relative electrical conductivity and a decrease on super oxide anion production rate, hydrogen peroxide during the whole growth stage. Simultaneity, O3 stress resulted in lower content of ascorbic acid and glutathione, and higher content of dehydroascorbic acid and oxidized glutathione of AsA-GSH cycle. The results showed that antioxidant system can not tolerate oxidative damage caused by elevated O3 concentration, thus increasing membrane lipid per oxidation, which represents injured effects to soybean.