Immobilization of lipase by filtration into a specially designed microstructure in the CA/PTFE composite membrane

摘要

Lipase (EC 3.1.1.3) is an important enzyme with a broad variety of industrial application due to the multiplicity of reactions it catalyzes. Due to their unique structural characteristics, Upases can catalyze reactions including organic substrates at the interface of organic and aqueous phases and can preserve their catalytic activity in organic solvents, biphasic systems and in micellar solutions. Application of lipase can be more economically and efficiently by immobilization to enhancing their activity, selectivity and operational stability. The immobilization of enzymes onto insoluble carriers is an important topic in enzyme technology and is fundamental for their application to industrial processes. Numerous efforts have been focused on the preparation of Upases in immobilized forms involving a variety of both support materials and immobilization methods.