Haemophilia A,one of the most common bleeding disorders,is the result of an inherited deficiency of Factor VIII,a protein involved in the blood coagulation cascade.For medical treatment,patients are given doses of Factor VIII derived from either blood plasma or recombinant cells.Factor VIII is usually concentrated by affinity chromatography,mostly employing monoclonal antibodies as ligands.In our work,we report the synthesis and optimization of an octapeptide sequence,which was the result of a screening for Factor VIII binders.Derived from the lead sequence,cyclic hexapeptides were synthesized and evaluated,resulting in new peptides with good Factor VIII binding properties as well as enhanced enzymatic stability.We also investigated the binding mode and the specifity of the binding.
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