An Experimental Study on Application of Immobilized Microorganism Technology for Treatment of Phenolic Wastewater

摘要

In this paper, phenol wastewater treatment was investigated by using improved PVA immobilization of good strains. Immobilization method based on the usage of phosphorylated polyvinyl alcohol (PVA) was developed. PVA was first cross linked with boric acid for a short time (10 min) to form a spherical structure, which was followed by solidification of the gel beads by esterification of PVA with phosphate. The optimum condition of immobilizing microorganism by PVA and the characteristics such as activity of immobilized gel, resistibility of high phenol were studied in the experiment. The optimum condition of immobilizing microorganism: the consistence of PVA is 100 g·L{sup}(-1); the PH of cross linker is 7.0. So the PVA hydrogel has a high tensile and folding strength and good handleability and processability. And the immobilized spherules utilized for phenol degradation after domestication are prepared by embedding immobilized bacteria sludge for degradation of phenol by the embedding carrier PVA, with the addition of feasible powder active carbon. The results show that the efficiency of degrading phenol by immobilized microorganism can be enhanced obviously. The degradation rate to 1500 mg·L{sup}(-1) can be beyond 98.3%. Moreover, the retaining time of wastewater has been shortened. This method has been proved to be an efficient approach to treat phenolic wastewater.