Monitoring Lymphatic Filariasis Interventions Through Adult Mosquito PCR Sampling in South Sumatra Province, Indonesia

摘要

Monitoring and evaluation are essential to the successful implementation of mass drug administration programs for lymphatic filariasis elimination. PCR-based methods are preferred over classical dissections with the protocol of one L3 Brugia malayi larva in a pool of mosquitoes. We analysed the partial HCL gene nucleotide sequence of the parasite inside the vector. Samples were collected with the methods of human landing, pyrethrum spray, and light traps catch, conducted concurrently at sites in an LF endemic Banyuasin district in South Sumatra. The DNA extraction method was DNAeasy Tissue kit (Quaigen Inc) were used on pools of 10 mosquitoes each. These were used as template DNA in PCR to amplify B. malayi sequences and thus sequenced. Our data showed a sequence of the filarial worm in Mansonia annulifera and Culex quinquefasciatus. To the best of our knowledge, this study is the first report of HLC gene nucleotide sequences from the species Cx. quinquefasciatus.