A Portable Fluorescent Microfluidic Detection Device Based on FPGA

摘要

Fluorescence analysis method has high sensitivity,but the difference between photoelectric converters will lead to inaccurate data.To solve the problem of the separation of effective and unwanted light in the process of fluorescence conversion and the noise of small-scale variable amplification.This paper designs a method of modulating the light source makes the fluorescent light be a kind of differential signal that is easily extracted by the photoelectric conversion circuit and amplified,and then uses digital devices FPGA and ADC to achieve signal acquisition and processing,and finally it is presented in the form of a dark room box.In this paper,a novel detection structure is used as a discussion point,and a low-power,high-precision,low-cost signal processing system has been introduced in detail,and mainly used in 500 The fluorescence in the wavelength range of-600 nm was tested.The mathematical relationship between different fluorescence intensities and the post-conversion variables was established.In the subsequent product development,the detection of different wavelengths of light can be achieved by replacing the excitation light source and the corresponding filter,providing a simple circuit processing method for designing photoelectric probes,biomedical detection,and chemical applications.