Research of Immobilization of Transglutaminase on Polypropylene Microporous Membranes

摘要

Activated ozone as the carrier, cryhc acid as the monomer and glutaraldehyde as the cross-linking agent were used to research the immobilization of transglutaminase on polypropylene microporous membranes. The effect of ozone activation time, grafting time, grafting temperature, monomer concentration and ammonium ferrous sulfate concentration on grafting degree were studied, the Immobilization conditions were also studied. We found the optimal reaction conditions as following: consistency of hexamethylendiamine was 15%, at 50°C for 120min and consistency, at 30°C for 45minites, consistency of enzyme was 15mg/mL at 4°C. So the immobilized amount of protein could come to 30.23mg/g membrane and the activity of immobilized MTG come to 16.9U/g membrane.