Purification and characterization of lipoprotein lipase from pseudomonas sp.

摘要

Lipoprotein Lipase (EC 3.1.13.) is the first enzyme in the series of enzymes used for determination of serun triglyceride in diagnositc kit. It is a surface-active enzymes becase binding to emulsified triglyceride substrates markedly increases their hydroloytic activity compared with their activity toward dissolved substrates. a procedure for the purification of a very hydrophobic lipase from Pseudomomas sp. by ammonium sulfate precipitation, Q-Sepharose chromatography, Octyl-Sephrose choromatography and Sephadex G-25 chromatography was described. The enzyme was purified 45 fold with a yield of 21