Lipase L1 Pseudomonas sp., method for obtaining it and sequences and grafts used in this method

摘要

in the disclosed enzyme lipase l1 having sequence seq id presented as well: 1. and the way to receive. lipase l1 is enzyme high activity lipolitycznej in the temperature range 20 - 120 degrees, the ph 8.5 to 9.5, and high compatibility with many types of detergents. also revealed plasmid containing dna sequence koduju0105cu0105 expressive weak lipase l1.the plasmid also contains the promoter transcription bakteriofaga t7 terminator, bakteriofaga t7 transcription, gene of resistance to kanamycin, laci gene, replication origin pbr322 and f1 replication origin. the plasmid sequence seq id may have no: 3. the subject of the invention is also rekombinantowy bacterium strain transformowany mentioned plasmid encoded.rekombinantowy bacterium strain is obtained from the following strains of escherichia coli: a tuner (de3), arctic express (de3).