Detection of Foreign DNA in Transgenic Fish with PCR technology

摘要

Recently, the study of fish as a model system of transgenic animals develops rapidly. (3.47). The main reason is that transgenic manipulation on fish embryos is relatively easier. For example, just microinjection of foreign DNA into occyte plasma of fertilized fish eggs may bring a highly effective integration. in mammal, howeer, the integration does not occur unless foreign DNA is injected into procaryon of ossperm. The advantage of transqenic manipulation on fish not only opens a new fiel for gene engineering, but also provides a conventient tool for the sudy of regulating eukaryotic genes. With the development of the research, some new problems arise-how to detct foreign DNA conveniently. It is discovered that microiniected foreign DNA can duplicate themselves in embryos and the maintained until the late stage of embryo development.