Identification of Avian Influenza Virus A/H5 Clade 2.3.2.1 in Asymptomatic-Ducks (Anas species) at a Live-Poultry Market in East Java, Indonesia

摘要

A total of 120 cloacal swab samples were collected from asymptomatic-ducks traded at a live-bird market in East Java-Indonesia during January to February 2017. After virus isolation using 10-days-old embryonated chicken eggs, hemagglutination activity was tested. TaqMan real-time reverse transcription (RT) polymerase chain reaction (PCR) assay was performed employing primer sets to differentiate HA genes of H5 clade 2.1.3 and clade 2.3.2.1. Our result show that Avian influenza virus (AIV)-A/H5 clade 2.3.2.1 was currently prevalent among ducks in a live-poultry market (LPM), indicating LPM could be an important place as an entry point of avian viruses to human resulting novel reassortant strain.