Research on Microbial Detection Technology Based on Femtosecond Laser Technology Raman Spectroscopy

摘要

Bacteria is a tiny microorganism that is inseparable from human life. Its rapid and efficient detection is essential for the prevention and control of major infectious diseases. Raman spectroscopy technology can provide relevant information about specific molecular structures, so it can be used to identify biochemical variations in tiny biological tissues. It is fast, nondestructive, and does not require pretreatment. It has great application prospects in pathological diagnosis and identification detection. Compared with conventional technology, Raman spectroscopy technology can directly detect living tissues, simplify analysis procedures, and effectively shorten diagnosis time. In this study, a combination of femtosecond laser technology and terahertz Raman spectroscopy was used for the detection of bacteria and microorganisms. The different bacterial groups of 100cfu and 1000cfu were respectively tested for Saccharomyces cerevisiae, Staphylococcus aureus, Salmonella paratyphi, and Spectral measurement of Candida albicans and Escherichia coli samples was carried out, and it was verified that this method has specific identification, quantitative characteristics and repetitive characteristics. The relative error of the detection results of the same flora at different concentrations has reached 6.0%, indicating that this method is applicable for the detection of different concentrations of the same bacteria and microorganisms.