Stain Transfer for Automatic Annotation of Malignant Lymphoma Regions in HE Stained Whole Slide Histopathology Images

摘要

We propose a method to transfer a given pathology image stained by some immunostaining to a H&E stained one. When one construct a classifier that estimates the subtype of malignant lymphoma from a given H&E stained pathology image, one needs a set of training H&E stained whole slide images in which the tumor regions are annotated. The annotation is not easy and requires large human resources. Here, it is known that some immunostaining stains only some specific tumor cells and the tumor region detection from the immunostained images is straightforward. It means once you transfer the immunostained images to H&E stained ones, you can easily obtain a set of virtually H&E stained images with annotation of tumor regions. In this manuscript, we report on the proposed method and experimental results of stain transfer from CD20 stained images to H&E stained ones.