Seeking for optimum culture medium for chlorella pyrenoidosa and analysis of the amino acid component

摘要

Chlorella Pyrenoidosa is one of the widely used algae in aquaculture. In order to find out a proper culture medium for high-density culturing to meet the demand of fish reproduction and water quality control, five normal algae culture media were test via multiple comparisons. The densities of the five groups, with six parallel samples each, which were cultured in corresponding medium, were determined by blood counting chamber on the beginning (0d), the 3rd day (3d), the 6th day (6d) and 9th day (9d). Then the growth curves were described and the growth radio were calculated and compared by One-Way ANOVA (Turkey method) using the SPSS 21.0 software. The multiple comparisons results revealed that HGZ group could reproduced a rapid and stable speed at high density, while SE group and Aquatic 4# reproduced faster than the HGZ group. Amino acid component results revealed that C. Pyrenoidosa contains 18 kinds of amino acid (42.19 ～ 43.14% of the body mass). There are significant differences on total amino acid, fish essential amino acid and non-essential amino acid between groups based on the multiple comparisons. However, the amino acid could not meet the demand of fish or shellfish. Thus, extra amino acid, such as tryptophan and methionine should be added and the HGZ medium was the best choice for producing C. Pyrenoidosa to satisfy the aquaculture.