The Blight-Resistance Gene Response to Bacterial Leaf Blight Disease in Isogenic Varieties through Pathogenicity Test

摘要

Bacterial Leaf Blight (BLB) disease is a one-factor cause of decreasing rice production in Indonesia. This disease is caused by Xanthomonas oryzaepv oryzae (Xoo), which infects rice leaves to yellowish. Xoo isolates were obtained from three different locations, namely West Java, West Sumatera, and West Borneo. Each isolate was identified by using primer specifics in colony Polymerase Chain Reaction (PCR) techniques. Isogenic strains that contained resistance genes were infected and observed through pathogenicity tests. Based on the test results, Code variety holding Xa4-Xa7 gene had a high resistance level on Xoo infection from all locations compared to other isogenic and popular rice strains. In the meantime, IRBB5 holding Xa5 gene survived the attack of Xoo from West Java and West Borneo properly. The infection intensities on Code and IRBB5 consecutively were 1.62% and 3.55% for West Java isolates, 2.10% and 2.97% for West Sumatera Isolates, 2.17% and 4.45% for West Borneo isolates.