Double core Infrared (CdSeTe)/ZnS quantum dots conjugated to Papiloma virus antibodies

摘要

The paper presents the photoluminescence (PL) study of the double core/shell infrared CdSeTe/ZnS quantum dots (QDs) in nonconjugated states and after the conjugation to the anti papiloma virus (i) mouse anti-HPV 16-E7 or ii) mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6) antibodies. CdSeTe/ZnS QDs with infrared emission at nearly 800 nm (1.6 eV), have been investigated. PL spectra of nonconjugated QDs are characterized by one Gaussian shape PL band related to the exciton emission in CdSeTe cores. Raman scattering spectra have been studied with the aim to reveal the CdSeTe double core composition. The Raman scattering study has shown that the central part of the core in QDs has the composition CdSe_(0.5)Te_(0.5) and the periphery part of the core has the composition CdSe_(0.7)Te_(0.3). PL spectra of bioconjugated QDs have changed: PL bands shift into the high energy and become asymmetric. The energy diagram of the double core/shell CdSeTe/ZnS QDs have been created for the nonconjugated QDs, which permits to explain the PL spectrum of nonconjugated QDs and its transformation at the bioconjugation to papiloma virus antibodies. It is shown that the PL spectrum transformation in bioconjugated QDs is promising for the study of the bioconjugation with specific antibodies and can be a powerful technique in biology and medicine.