Biodegradable Amphiphilic Tri-block Copolymeric Nanoparticles for Controlled MTB Drug Delivery

摘要

A series of biodegradable amphiphilic tri-block copolymers (PLGA-PEG-PLGA) have been derived from the di-block copolymer poly (lactic-co-glycolic acid (PLGA)) and polyethylene glycol (PEG). The mycobacterium tuberculosis (MTB) drag pyrazinamide (PZA) loaded polymer nanoparticles (NPs) have been prepared by probe-sonication followed by w/o/w double emulsification technique. The copolymers have been characterized by FTIR and 'HNMR spectroscopic techniques, TG-DTA analysis, GPC analysis and powder XRD pattern. The MTB drag loaded polymeric NPs have been characterized by FESEM, powder XRD, HRTEM and XPS analysis. The drag loading efficiency, drag content and in vitro drag release studies have been carried out by spectrophotometry. The drug loading efficiency and drug content of triblock copolymeric NPs were higher than these of diblock copolymeric microparticles (MPs). The in vitro drag release studies indicate that the NPs exhibit initial burst release followed by controlled release of PZA for longer durations. The drag release kinetics mechanism has been evaluated by zero order, first order, Korsemeyer-Peppas (KP) and Higuchi models.