Multimodal measurements of brain tissue metabolism and perfusion in a neonatal model of hypoxic-ischaemic injury

摘要

This is the first multimodal study of cerebral tissue metabolism and perfusion post-hypoxic-ischaemic (HI) brain injury with broadband near-infrared spectroscopy (bNIRS), diffuse correlation spectroscopy (DCS), positron emission tomography (PET) and magnetic resonance spectroscopy (MRS). In 5 piglet models of HI, we measured cerebral tissue saturation (StO_2), cerebral blood flow (CBF), cerebral oxygen metabolism (CMRO_2), changes in the mitochondrial oxidation state of cytochrome-c-oxidase (oxCCO), cerebral glucose metabolism (CMRglc), and tissue biochemistry (Lac+Thr/tNAA). At baseline, the parameters measured were: 64+6 % StO_2, 35±11 ml/100g/min CBF, and 2.0±0.4 μmol/100g/min CMRO_2. After HI the parameters measured were: 68±6% StO_2, 35±6 ml/100g/min CBF, 1.3±0.1 μmol/100g/min CMRO_2, 0.4±0.2 Lac+Thr/tNAA, and 9.5+2.0 CMRglc. This study demonstrates the capacity of a multimodal set up to interrogate the pathophysiology of HIE using a combination of optical methods, MRS, and PET.