Optical microscopy using annular full-color LED for quantitative phase and spectroscopic imaging of biological tissues

摘要

We developed an optical microscope using oblique illumination and active image processing, which has the advantages of separate phase and amplitude contrast imaging, reduction of multi-scattering effects in biological tissues, and spherical aberration-free imaging. This microscope imaged tissue cells by processing oblique illumination images in a Fourier space taken from all directions azimuthally. In this research, a ring-shaped annular full-color RGB LED light source was developed to attain high-speed imaging and to obtain spectroscopic and quantitative phase structures of biological samples. Reconstructive imaging of a pine pollen structure captured with the annular full-color LED shortened the measurement time and obtained absorption and phase-contrast images for each RGB color.