Cellular internalization and intratumoral distribution of prospectively therapeutic magnetic nanoparticles clusters submitted or non-submitted to pulsed electric fields

摘要

The intracellular localization and the (therapeutic) effects of nanoparticles might depend on the processes by which the nanoparticles had entered the cell. In order to evaluate different internalization patterns, cultured cells have been incubated with superparamagnetic iron oxide nanoclusters. Different incubation protocols have been applied, including the application of sub-permeabilizing and permeabilizing pulsed electric fields, and the efficiency and impact of the applied cell labeling protocol has been assessed.