Gas phase spectroscopy of complexes of a partial peptide of adrenoceptor and analogs of adrenaline by using an electrospray cold ion trap technique

摘要

Neurotransmitters is bound to specific receptors molecular-selectively. To understand these processes, not only how proper ligands bind to the receptors, but also why non-proper ligands cannot bind should be clarified. By using X-ray diffraction method, we can know how the proper ligand is bound in the receptor at atomic resolution. Non-proper ligand is, however, difficult to be crystallized with the receptor, thus it is difficult to get why the non-proper ligand is rejected. Here, we tried to interrogate the structure of the complex of several (proper and non-proper) ligands and Ac-Ser-Ile-Val-Ser-Phe-NHCH3 (SIVSF) peptide, which is a binding motif of β2-adrenoceptor, by using gas phase spectroscopic technique in combination with electrospray and cryogenic ion trap techniques (Fig.1).1